In Africa, Cassava mosaic disease is a leading constraint on the production of cassava (Manihot esculenta), a tuberous root crop plant originally from the Americas. The resilience of cassava to withstand droughts and grow in diverse ecological locations has resulted in cassava being the main source of income for many developing countries and the basic diet for about 500 million people worldwide. Cassava is infected by a complex of geminiviruses that are characterized by small, single-stranded DNA genomes composed of A and B components. The A component controls replication using the host machinery and the B component controls movement. Recently, “Satellite Like” molecules (SAT) were shown to break endogenous resistance to geminiviruses in cassava. We wanted to test whether the SAT molecules also break engineered resistance against geminiviruses using transgenic Nicotiana benthamia plants that are resistant Tomato golden mosaic virus (TGMV). To analyze the SATs’ abilities to break TGMV resistance we inoculated transgenic plants with Agrobacterium vectors containing TGMV and the SATs. In addition, to see if the SATs effect replication of TGMV we used Agrobacterium vectors containing the SATs and the A component in leaf disc replication assays. Due to low efficiency of viral infection in nontransgenic, control plants, our data did not allow us to conclude whether the SATs can break TGMV-resistance. The infection and replication studies are being repeated to determine the effect the SATs have on TGMV infection.