The functionalization of nanoparticles has many applications, specifically in biosensing devices. In this project, the surfaces of biosensors are coated with amine, alkane, or carboxyl functionalized gold nanoparticles. The surface potential of the functionalizations electrically gate the electrons in the channel of the biosensor, changing the electrical properties of the device. This surface potential is measured using Kelvin Probe Force Microscopy (KPFM). By understanding these electrical properties, nanoparticle coated biosensors can be modified to have quick response, high specificity, inexpensive fabrication, reusability, multiplexing, and label-free detection. The results acquired from Kelvin Probe were statistically significant and the functionalizations had an effect on the surface potential of the nanoparticles compared to the control experiment where functionalizations were stripped with UV light.