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2012 - 11th Annual NC State Summer Undergraduate Research Symposium
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Session Time :
8/1/12 3:00 PM - 8/1/12 4:14 PM
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Content Area : NC State Independent Researchers
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Lead Student Presenters : Yan Ting Mak
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Abstract Title : Efficacy of Salmonella Detection in Poultry: Evaluating Different Sampling Methods to Minimize Number of Animal Use
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Abstract :
Under normal experimental protocols, researchers need to repeatedly euthanize an overwhelming amount of animals to frequently obtain caecal samples to test for the persistence of Salmonella Typhimurium. To be humane and cost efficient, it would be beneficial to determine an alternative method for collecting cecum samples that did not require the euthanization of the chicks. In the first trial, we explored the correlation between cecum samples and cloacal swabbing samples, where samples of the cecum and cloacal swabs were plated on XLT4 media containing the appropriate antibiotics to phenotypically select for Salmonella colonies. All viable counts were later confirmed by API diagnostic strip tests to be indeed Salmonella Typhimurium. There was a weak correlation found for trial 1(R2 value= 0.098), however, cloacal swabs tend to be able to detect presence of Salmonella only when there were high concentrations of Salmonella in cecum samples. To further track the presence of Salmonella in the chick’s digestive tract and search for other correlative factors, trial 2 of the research included sampling from the colon as well. Trial 2 has shown greater R2 values in “colon verses cecum” samples (overall R2 value= 0.5326) than “colon verses cloacal swab” samples (overall R2 value= 0.1702) and “cecum verses cloacal swab” samples (overall R2 value=0.2095) as expected. This elucidates the ineffectiveness of cloacal swabbing for detecting the presences of Salmonella Typhimurium in chickens.
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Mentor and/or Co-Author : Hosni Moustafa Hassan
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