Hosni M. Hassan
Professor of Microbiology, Biochemistry, Food Science, and Toxicology
hmhassan@ncsu.edu
 
EDUCATIONAL BACKGROUND
B.Sc. (1959) Food Science and Microbiology – Ain Shams University, Cairo, Egypt
Ph.D. (1967) Microbiology – University of California, Davis, USA
Postdoctoral Fellow (1967-1968) – Microbiology - University of California, Davis, USA
 
ACADEMIC and PROFESSIONAL EXPERIENCE
July 2013 – Present      Professor of Microbiology, Biochemistry, Food Science, Toxicology and the Prestage Department of Poultry Science, NCSU, Raleigh, NC 27695-7608
July 1984- Present   Professor of Microbiology, Biochemistry, Food Science, Toxicology and member of the Biotechnology faculty, NCSU,     Raleigh, NC 27695-7615
Sept. 1993 – June 2004   Professor and Head, Department of Microbiology, NCSU; and Interim Head of the Department of Environmental and Molecular Toxicology, NCSU from November 1999 to March 2001   
Aug. 1980 - June 1984  Associate Professor Departments of Food Science, Microbiology and Toxicology, NCSU, Raleigh, NC 27695-7624
Sept. 1979 - Aug. 1980  Associate Professor Department of Microbiology and Immunology McGill University ? Medical School Montreal, Quebec H3A 2B4 -Canada
Sept. 1975 -  Aug. 1979  Research Associate, Department of Biochemistry, Duke University Medical Center Durham, North Carolina 27710
 
COURSES TAUGHT
Current Issues in Microbiology (1 Cr.) - MB 480 (2009- 2013)
Free Radicals in Toxicology (1 Cr.) - TOX (BCH) 606 (1986- 2010)
Lectures (3) in Environmental Toxicology (3 Cr.) - TOX 515 / 710 (1985- 1997)
Microbial Physiology - MB 501A (1982-1987)
Food Microbiology (4 Cr.) - MB 405/FS 405 (1991)
Microbiology Research - MB 699 (1980-present)
Advanced Food Microbiology (3 Cr.) - FS 506 (1982-1984)
Microbial Toxins (3 Cr.) - FS 591B (1985-1991)
Biochemical Metabolism & Mol. Biol. (3 Cr.) - BCH 453 (1992)
 
FACULTY MEMBERSHIPS
Poultry Science
Microbiology
Biotechnology
Toxicology
Biochemistry
Food Science
 
RESEARCH INTERESTS
During the past thirty five years, I have been working on oxidative stress and microbial antioxidant enzymes (i.e., Superoxide Dismutases and Hydroperoxidases).  We established that superoxide radical (O2?) is a common product of biological oxygen reduction, and that superoxide dismutases (SODs) and Hydroperoxidases (catalases/peroxidases) constitute essential defenses against the toxic effects of oxygen free radical.  We demonstrated that the synthesis of SOD is responsive to changes in pO2, the specific growth rate of the organism, and to the rate of oxygen uptake.  We also elucidated the molecular mechanisms for the regulation of sodA in E. coli by four repressors [Fur, ArcA/B, FNR, and IHF] and one aerobic activator [SoxRS]. We are also interested in the cloning and expression of heterologous antioxidant genes in the probiotic lactic acid bacteria, which normally lack these genes, for use in the treatment of inflammatory bowel diseases, e.g., Crohn's disease, and for protection against therapeutic and terrorism-mediated radiation damages of the GI tract of human.
Currently, work is in progress to determine how the global regulators (i.e., Fur, Fnr and ArcA) coordinate the expression of antioxidant enzymes, cellular metabolism, and virulence in Salmonella Typhimurium and other pathogens.  The findings led us to develop and patent a certain attenuated live vaccine strain for protection against Salmonellosis and possibly other Food-borne pathogens. Studies are in progress to develop a novel Salmonella control strategy that promotes the establishment of an intestinal microbiota in the chicken and other animals  that prevents colonization by pathogenic bacteria and enhances the mucosal immune response to Salmonella vaccination and challenges by virulent strains.
 
CURRENT FUNDING (Principal Investigator/Program Director)

USDA-NIFA Integrated grant: Development of novel Salmonella control practices and integrated education program to reduce Salmonellosis – H. Hassan (PI); M. Koci, S. Perry, and A. Chilcote (CoPIs). $2,500.000.00 (2012-2017)
 
SELECTED PUBLICATIONS
Dobrogosz, W. J., T. J. Peacock, and H. M. Hassan (2010). Evolution of the probiotic concept: from conception to validation and acceptance in medical science. Adv. Appl. Microbiol. 72:1-41.
Troxell, B., M.L. Sikes, R.C. Fink, A. Vazquez-Torres, J. Jones-Carson, and H. M. Hassan. (2011). Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar Typhimurium. J. Bacteriol. 193: 497-505.
Evans, M.R., R.C. Fink, A. Vanquez-Torres, S. Porwollik, J. Jones-Carson, M. McClelland, and H. M. Hassan.  (2011). Analysis of the ArcA regulon of Salmonella enterica sv. Typhimurium 14028s. BCM Microbiology  11:58  (doi:101186/1471-2190-11-58)
Troxell, B., R.C. Fink, S. Porwollik, M. McClelland, and H. M. Hassan. (2011). The Fur Regulon in Anaerobically Grown Salmonella enterica sv. Typhimurium: Identification of New Fur Targets. BCM Microbiology 11:236 (doi:101186/1471-2180-11-236)
Qiu, R.,  J. Croom, R.A. Ali, C. Ashwell, H. M. Hassan, C.C. Chiang, and M.D. Koci. (2012). Direct fed microbial supplementation repartitions host energy to the immune system. J. Animal Sci. 90:1-13 (doi:10.2527/Jas 2011-4611)
Troxell, B and H.M. Hassan (2013) Transcriptional regulation by Ferric Uptake Regulator (Fur) in pathogenic bacteria. Front. Cell. Infect. Microbiol3:59. doi: 10.3389/fcimb.2013.00059
Husain, M., J. Jones-Carson, L. Liu, M. Song, J. R. Saah, B. Troxell, M. Mendoza, H. Hassan, and A. Vázquez-Torres (2013). Ferric uptake regulator-dependent antinitrosative defenses in Salmonella pathogenesis. Infec. Immun.  doi:10.1128/IAI.01201-13
 
PROFESSIONAL AFFLIATIONS
American Society for Biochemistry and Molecular Biology
American Society for Microbiology
The New York Academy of Sciences
International Society for Free Radical Research
Oxygen Society  
Fellow of the American Institute of Chemists
Sigma XI
Phi Tau Sigma
 
Patents:         
Attenuated FNR Deficient Enterobacteria -U.S. Patent No: 8,435,506 – Issued May 7, 2013
Attenuated FNR Deficient Enterobacteria-U.S. Patent No: 8,101,168 – Issued Jan. 24, 2012
Vacuum Drum Planters - U.S. Patent N0: 4,306,509 – Issued December 22, 1981
 
HONORS AND AWARDS
Fulbright Senior Research Scholar, Jacques Monod Institute, University of Paris VII, Academic Year 1987-1988
President-elect and President of NC branch of American Society for Microbiology, 1993 & 1994