James N. Petitte
A.B. (1979) Susquehanna University, Pennsylvania
M.S. (1981) University of Maine, Orono, Maine
Ph.D (1986) University of Guelph, Guelph, Ontario
NCSU Physiology Graduate Program
Center for Comparative Medicine and Translational Research
Chickens, turkeys, and ducks supply about 25-30% of the animal protein consumed in the world. This statistic is the result of an 80-year transformation of the poultry industry from backyard flocks to a highly sophisticated, efficient, and economically important industry organized on an international scale. Much of the growth and development of the poultry industry can be attributed to the application of the principles of contemporary biology to genetic selection programs, to the immunology of disease control, and to nutrition programs needed to maintain high levels of production.
The major emphasis of the current research program is on the development of a novel method of gene transfer for the chicken and the application of avian biotechnology to biomedicine and agriculture. Recent work on the production of germline chimeras suggests that the use of cells from the early blastoderm as well as isolated primordial germ cells could be used to insert DNA into the avian germline. The culture of avian embryonic stem cells and primordial germ cells is now under investigation using several approaches. In addition, investigations on the origin of primordial germ cells in the developing embryo offer additional clues to the development of transgenic poultry.
Anderson KE, Petitte J, Whitaker R, Lancaster JM, Wenham RM, Carver DK, Turbov J, Berchuck A, Kopelovich L, Rodriguez GC.Ovarian adenocarcinomas in the laying hen and women share similar alterations in p53, ras, and HER-2/neu. 2009. Cancer Prev Res (Phila Pa) 2(2):114-21. Epub 2009 Jan 27.
Ge, C, Yu, M., Petitte, J.N. and Zhang, C. 2008. Epidermal Growth Factor-Induced Proliferation of Chicken Primordial Germ Cells: Involvement of Calcium/Protein Kinase C and NFKB1. Biology of Reproduction, 80(3):528-36. Epub 2008 Nov 12.
Petitte J.N. and P.E. Mozdziak. 2007. The incredible, edible, and therapeutic egg. PNAS 104: 1739-1740
Cho J., K. Choi, T. Darden, P.R. Reynolds , J.N. Petitte , S.B. Shears. 2006. Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's "phosphate crisis". J of Biotechnology. 126(2):248-59.
Mozdziak, P.E., B. Wysocky, J. Angerman-Stewart, S.L. Pardue, and J.N. Petitte 2006. Production of chick germline chimeras from FACS sorted embryonic gonocytes. Poultry Science, 85(10):1764-8.
HONORS AND AWARDS (INCL PATENTS)
Mozdziak, P.E. and Petitte, J.N. Transgenic reptiles. Issued March 13, 2012; US Patent #8,134,044
Petitte, J.N. and Pardue, S.L. Depletion of endogenous primordial germ cells in avian species Issued August 9, 2011;7,994,388
Mozdziak, P.E. and Petitte, J.N. Transgenic snakes and methods of making. Issued: February 16, 2010; US Patent # 7,663,019
Petitte, J.N. and Zhang, Y.G. Culture of undifferentiated chicken cells. Issued: September 9, 2008; US Patent # 7,422,897
Pardue, S. L., Petitte, J. N., D'Costa, S. and Song, Y. Methods for Gamete Production in Birds. Issued February 17, 2004; US Patent # 6,691,638
Petitte, J.N., Ricks, C.A., Spence, S.E. Gene transfer in poultry by introduction of embryo cells in ovo. Issued: February 4, 2003; US Patent #6515199
Petitte, J.N., Ricks, C.A., Phelps, P.V., and Williams, C. Gene transfer in chickens by introduction of DNA into muscle in ovo . Issued: May 28, 2002; U.S. Patent # 6395961
Pardue, S. L., Petitte, J. N., and D'Costa, S. Methods for Gamete Production in Birds. Issued: March 12, 2002; U.S. Patent #6,354,242
Petitte, J. N. and Chang, I. Method of Producing an Undifferentiated Avian Cell Culture using Avian Primordial Germ Cells. Issued: December 25, 2001; U.S. Patent # 6,333,192.
Petitte, J. N. and Yang, Z. Veterinary Pharmaceutical Formulation Containing Avian Embryonic Stem cells. Issued: November 3, 1998; U.S. Patent # 5,830,510.
Petitte, J. and C. A. Ricks, Apparatus for Injecting Avian Embryo Muscle Tissue In Ovo, Issued: July 28, 1998, U.S. Patent # 5,784,992.
Petitte, J. N. and Yang, Z. Avian Embryonic Stem Cells. Issued: August 12, 1997; U.S. Patent # 5,656,479.
Petitte, J. N. and Yang, Z. Method of Producing an Avian Embryonic Stem Cell Culture and the Avian Embryonic stem Cell culture Produced by the Process. Issued: August 23, 1994; U.S. Patent # 5,340,740.