USDA-FAS Borlaug Felloship Program researching “ Weaken wheat phenylalanine ammonia-lyase (PAL) gene promotor by Homology Directed Repair (HDR) CRISPR-Cas9 technology to obtain more economical and effective biofuiel production”. The training activitives include literature studies to learning plant phenylpropaoid pathway, CRISPR/Cas9, and CRISPR/Cpf1, training in molecular biology and bioinformatics, promoter sequence analysis, identification of protospacer adjacent motif (PAM), and development callus culture of bread wheat, expression profiling of 37 PAL genes in bread wheat tissues, CRISPR/Cas9 and CRISPR/Cpf1 construct developments, genetic transformation of wheat callus and validation of gene editing. By the end of this program, the fellow, Ekin Demiray, who will be working with Dr. Deyu Xie, will gain sufficient training to learn how to develop genome editing research back to their own campus.