Bio-molecular Interaction facility



Surface Plasmon Resonance & Biolayer Light Interferometry

Biacore 3000: Surface Plasmon Resonance - is a system for real-time biomolecular interaction analysis using surface plasmon resonance (SPR) technology. SPR is a non-invasive optical measuring technique which measures the mass concentration of biomolecules in close proximity to the specially prepared surface.

BLItz: Biolayer Light Interferometry - alternative technique to SPR that is "free from microfluidics" - Label free determination of protein presence/absence instantly, binding kinetics, quantitation of proteins, and immunoassays

Ultra pressure liquid chromatography:

Ultra performance liquid chromatography - achieve equal or better resolution LC separations in much shorter time frames

Circular Dichroism

Circular Dichroism - Analyzes secondary structure or conformation of macromolecules. CD observes how secondary structure changes with environmental conditions or on interaction with other molecules. Structural, kinetic and thermodynamic information about macromolecules can be derived from circular dichroism spectroscopy.

Analytical Ultracentrifugation:

Proteome Lab XL-A: Analytical Ultracentrifugation (AUC) is a non-destructive technique used to assess the size and shape of macromolecules. It can also be used to assess multimerization states and binding constants for intermolecular interactions.